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Oxydative stress in rats caused by coal dust plus cigarette smoke

Nia Kania, Bambang Setiawan, H.M.S. Chandra Kusuma
Submission date: Tuesday, 23 February 2016
Published date: Tuesday, 23 February 2016
DOI: http://dx.doi.org/10.18051/UnivMed.2011.v30.80-87

Abstract


Coal dust and cigarette smoke are pollutants found in coal mines that are capable of inducing oxidative stress, the effects of which on blood malondialdehyde (MDA) level and serum superoxide dismutase (SOD) level are still unknown. The purpose of the present study was to evaluate the effect of coal dust and cigarette smoke on levels of MDA and SOD in rats. An experimental study was done on Wistar male rats divided into the following groups: control (C), coal dust exposure (14 days) (CDE), cigarette smoke exposure (14 days) (CSE), coal dust exposure (7 days) followed by cigarette smoke exposure (7 days) (CDE+CSE), cigarette smoke exposure (7 days) followed by coal dust exposure (7 days) (CSE+CDE). All exposures increased MDA levels and decreased SOD activity significantly between groups (p=0.000). All exposure groups had significantly increased blood MDA levels, compared to the control group, although there was no difference between CSE + CDE and CDE + CSE. For SOD levels, all exposure groups had significantly decreased the SOD levels compared to control. But there were no significant differences between CSE vs CDE and CDE + CSE vs CSE + CDE. We conclude that exposure to cigarette smoke significantly increases blood MDA level and decreases serum SOD activity, which was not found in exposure to coal dust. Combined exposures also increase blood MDA level and decrease serum SOD activity significantly.

Keywords


Coal dust; cigarette smoke; malondialdehyde; superoxide dismutase; rats

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References


Huang X, Finkelman RB. Understanding the chemical properties of macerals and minerals in coal and its potential application for occupational lung disease prevention. J Toxicold Environ Health Part B 2008;11:45-67.

Huang X, Li W, Attfield MD, Nadas A, Frenkel C, Finkelman RB. Mapping and prediction of coal workers’ pneumoconiosis with bioavailable iron content in the bituminous coals. Environ Health Perspect 2005;113:964–8.

Hendryx M, Zullig KJ. Higher coronary artery disease and heart attack morbidity in Appalachian coal mining regions. Prev Med 2009;49:355-9.

Lykkesfeldt J, Svendsen O. Oxidant and antioxidants in disease: oxidative stress in farm animals. Vet J 2007;173:502-11.

Glorie G, Legrand-Poels S, Piette J. NF-kB activation by reactive oxygen species: fifteen years later. Biochem Pharmacol 2006;72:1493-505.

Romieu I, Castro-Giner F, Kunzli N, Sunyer J. Air pollution, oxidative stress and dietary supplementation: a review. Europ Respir J 2008; 31:179-96.

Pinho RA, Silveira PCL, Silva LA, Steck EL, Dal-Pizzol F, Moreira JCF. N-acetylsisteine and deferoxamine reduce pulmonary oxidative stress and inflammation in rats after coal dust exposure. Environ Res 2005;99:355-60.

Halliwell B, Whiteman M. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Pharmacol 2004;142:231-55.

Armutcu F, Gun BD, Altin R, Gurel A. Examination of lung toxicity, oxidant/antioxidant status and effect of erdosteine in rats kept in coal mine ambience. Environ Toxicol Pharmacol 2007;24:106-13.

Junior SA, Possamai FP, Budni P, Backes P, Parisotto EB, Rizelio VM, et al. Occupational airborne contamination in south Brazil: oxidative stress detected in blood of coal miners. Ecotoxicology 2009;18:1150-7.

Ulker OC, Yucesoy B, Demir O, Tekin IO, Karakaya A. Serum and BAL cytokine and antioxidant enzyme level at different stages of pneumoconiosis in coal workers. Human Exper Toxicol 2008;27:871-7.

Murarescu ED, Iancu R, Mihailovici MS. Morphological changes positive correlates with oxidative stress in COPD. Preliminary data of an experimental rat model-study literature review. Romanian J Morphol Embryol 2007;48:59-65.

Stell, RGD, Torrie H. Principles and procedures of statistics, a biometrical approach 2nd Ed. Singapore: McGraw Hill Book Company;1991.

Ghanem MM, Porter D, Batteli LA, Valyathan V, Kashon ML, Ma JY, et al. Respirable coal dust particle modify cytochrome P4501A1 expression in rat alveolar cells. Am J Respir Cell Molecular Biol 2004;31:171-87.

Xi YD, Hou XM, Hong Y, Wei LX, Ke Z, Chang W, et al. Physicochemical properties and potential helath effects of nanoparticles from pulverized coal combustion. Chine Sci Bull 2009;54:1243-50.

Donaldson K, Tran L, Jimenezi LA, Duffin R, Newby DE, Mills N, et al. Combustion-deroved nanoparticles: a review of their toxicology following inhalation exposure. Part Fibre Toxicol 2005;2:10.

Simeonova PP. Nanoparticle exposure and systemic/cardiovascular effects-experimental data. Nanotechnol Toxicol Issues Environ Safety 2007:53-64.

Brook RD, Rajagopalan S, Pope A, Brook JR, Bhatnagar A, Diez-Roux AV, et al. Particulatte matter air pollution and cardiovascular disease: an update to the scientific statement from the American Heart Association. Circulation 2010; 121:2331-78.

Gregory CD, Pound JD. Microenvironmental influences of apoptosis in vivo and in vitro. Apoptosis 2010;15:1029-49.

Biswas SK, Rahman I. Environmental toxicity, redox signalling, and lung inflammation. Mol Aspect Med 2009;30:60-76.


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